Bonhams is auctioning off the British-made classic car at their Goodwood Festival of Speed Sale in Chichester, England on July 13.
The Guinness World Record-setting coupe became the most valuable piece of Bond memorabilia ever sold when it was purchased by its current owner in 2001. It's expected to fetch $1.4-$1.9 million.
The DB5 is widely regarded 007's most iconic ride, having made its glorious debut alongside Sean Connery in 1964's Goldfinger.
This particular model is the exact same one Pierce Brosnan raced against a Ferrari F355 piloted by femme fatale Xenia Onatopp (aka Famke Janssen) in the 1995 Bond installment.
Anybody who saw Daniel Craig's portrayal of the suave MI6 agent in 2012's Skyfall at the movies will remember the eruption of applause that broke out when another glistening 1965 Aston Martin DB5 made a surprise appearance.
Sholto Gilbertson, Bonhams Motor Cars Department Director, said "The DB5 is one of the most recognizable and desirable British classic cars in the world—it is also the ultimate Bond car."
"Every fan remembers Pierce Brosnan tearing through the hills in this car, and it must be up there as one of the most thrilling Bond car chases in history. This Aston Martin is something very special indeed."
h/t: New Atlas